Diabetes is toe te schrijven aan gemengde afwijkingen in de insulineproductie en beweging. De pathofysiologie van deze defecten is uitgebreid bestudeerd en redelijkerwijs begrepen.
Hun oorzaken zijn ongrijpbaar en hun manifestaties zijn pleiotroop, waarschijnlijk als gevolg van het drievoudige risico van genen, omgeving en levensstijl. Behandelaars brengen, zodra ze zich beperken tot monotherapie met secretagogen of insuline, nu geavanceerde mengsels van kostbare behandelingen die de ontwikkeling afremmen, maar die in wezen de onderliggende oorzaken van de ziekte niet veranderen.
Naarmate de vooruitgang in ons begrip van insulinebeweging en β-celfalen een essentieel stadium bereikt, put ik hier uit lessen die zijn gerealiseerd uit onze analyse van insulineregulatie van genexpressie en pancreatische β-celdifferentiatie om de vraag af te handelen van hoe we deze spannende biologie zullen vertalen in op mechanismen gebaseerde interventies om het beloop van diabetes om te keren.
Insulin Action Research and the Future of Diabetes Treatment: The 2017 Banting Medal for Scientific Achievement Lecture.
Analyse van diabetes mellitus-determinanten in Indonesië: een onderzoek uit het Indonesische basisgezondheidsonderzoek 2013.
ACHTERGROND diabetes mellitus is een stille moordenaar. De prevalentie en indruk op welzijnsrekeningen nemen toe van 12 maanden tot 12 maanden.
Dit onderzoekt doelen om de eigenschappen en de bedreigingscomponenten te analyseren die een effect hebben op diabetes mellitus in Indonesië. METHODE Dit is een cross-sectioneel onderzoek. Gegevens zijn verkregen uit het Basisgezondheidsonderzoek (RISKESDAS) in 2013.
De monsters zijn mensen van ≥15 jaar oud, bij wie de nuchtere bloedglucose en 2 uur bloedglucose na het opleggen zijn gemeten. Voor dit onderzoek zijn 38.052 personen gekozen. De variabelen leeftijd, geslachtsgemeenschap, burgerlijke staat, scholingsstadium, beroepsstatus, woonruimte, regionale status, hypertensie, gewichtsproblemen, rookgedrag en dyslipidemie worden geanalyseerd als bedreigingscomponenten voor diabetes mellitus.
Description: Non-RI dependent diabetes, or Type 2 Diabetes, is characterized by hyperglycemia that is caused by a combination of RI resistance and the body's s inability to compensate by producing more RI.
Description: Type I Diabetes peripheral blood plasma is obtained by centrifugation of the Type I Diabetes PB. This product has a diminished number of cells and platelets. Fresh Diabetic Type I PB plasma is available upon request.
Description: Type II Diabetes peripheral blood plasma is obtained by centrifugation of the Type II Diabetes PB. This product has a diminished number of cells and platelets. Fresh Diabetic Type II PB plasma is available upon request.
Description: Diabetic Type I and Type II HSMM-Human Skeletal Muscle Myoblasts are isolated from donors diagnosed with either Diabetes Type I or Diabetes Type II. Additional donor information is available by contacting Scientific Support.
Description: Diabetic Type I and Type II HSMM-Human Skeletal Muscle Myoblasts are isolated from donors diagnosed with either Diabetes Type I or Diabetes Type II. Additional donor information is available by contacting Scientific Support.
Description: Induced Pluriopotent Stem Cells (iPSCs) are a type of pluripotent stem cells that can be derived directly from adult somatic cells . The derived iPSCs can propagate indefinitely, as well as give rise to other cell types in the body. iPS cells, thus, hold great promise in the field of regenerative medicine by representing a single source of cells that could be used to replace those damaged/diseased cells.
Description: Peripheral blood mononuclear cells are isolated from Type I Diabetes peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation,the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Fresh Diabetic Type I PBMCs is available upon request.
Human Type II Diabetes Peripheral Blood Mononuclear Cells
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell is defined as any blood cell with a round nucleus. These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.Samples from each donor are tested via PCR to confirm non-reactivity. Peripheral blood mononuclear cells are isolated from Type II Diabetes peripheral blood by diluting the whole blood with PBS and using gradient separation techniques. After centrifugation,the mononuclear cell layer is collected. Mononuclear Cells can be processed further to isolate subpopulations. Fresh Diabetic Type II PBMCs is available upon request.
HighQC™ Human IPS Cell (Episomal/ Diabetes Type II/ Adipose)
Description: Gentaur offers human iPS cell lines derived from PBMCs, dermal fibroblasts, or adipose tissue of patients with Parkinson's Disease (PD) and Diabetes II. These iPS cells are established from a single clone and expanded in feeder-free conditions.
HighQC™ Induced Pluripotent Stem Cell, From Diabetes (mellitus)
Description: Diabetes (mellitus), Primary cell type: Fibroblast, Unknown vector detectable; Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.
HighQC™ Human IPS Cell (IPSC From PBMC/ Diabetes Type II/ Episomal)
Description: Gentaur offers human iPS cell lines derived from PBMCs, dermal fibroblasts, or adipose tissue of patients with Parkinson's Disease (PD) and Diabetes II. These iPS cells are established from a single clone and expanded in feeder-free conditions.
Rrad (GFP-tagged) - Mouse Ras-related associated with diabetes (Rrad)
Description: Gentaur is is proud to offer human iPS cell lines derived from the human dermal fibroblasts from patients with Type 2 Diabetes (T2D). The pertinent donor information is available upon request. These iPS cells are established from a single clone and expanded in feeder-free conditions. Normal human iPS cell lines are also available as separate products . We also provide custom iPSC generation and iPSC differentiation services to meet your needs.
Rrad (untagged ORF) - Rat Ras-related associated with diabetes (Rrad), (10 ug)
Description: Human MSCs (Bone Marrow Derived) from a single adult donor clinically diagnosed with Type II Diabetes. The MSCs are compatible with our MSC Expansion Medium for Bone Marrow Derived MSCs.All of the cells provided are tested and are negative for HIV-1, HIV-2, Hepatitis B and Hepatitis C as detected by PCR. The cells are confirmed to be negative for mycoplasma and other detectable microbial contamination.
RRAD (untagged)-Human Ras-related associated with diabetes (RRAD), transcript variant 1
Description: Human lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human lung tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human small intestine: Ileum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine: Ileum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine: Ileum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine: Ileum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human small intestine: Jejunum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine: Jejunum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine: Jejunum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine: Jejunum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human stomach tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human stomach tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the stomach tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The stomach tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human duodenum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human duodenum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the duodenum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The duodenum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human esophagus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human esophagus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the esophagus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The esophagus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human pancrease tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancrease tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancrease tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancrease tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: human skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human skeletal muscle tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the Skeletal Muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The Skeletal Muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Gentaur epidermal keratinocytes are available from adult and neonatal donor tissue and are provided at the earliest passage without the use of a murine feeder layer. Keratinocytes are available from donors of varying ages, genders, and diabetes status. Donor matched epidermal keratinocytes, dermal fibroblasts, melanocytes and subcutaneous preadipocytes are available.
Description: Mesothelial cells play pivotal roles in ovarian cancer metastasis, peritoneal dialysis, surgical adhesions, inflammatory response, and metabolic disease. These specialized epithelial cells form a single cell layer with a critical barrier function and provide a frictionless surface for organs and tissue to move without damage. We isolate peritoneal mesothelial cells from omental tissue biopsies with minimal propagation.
cDNA - Human Diabetic Diseased Tissue: Bone Marrow
Description: Human pancreas tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human pancreas tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated pancreas tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated pancreas tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Human Adipose Derived Stem Cells (ADSC) from normal (non-diabetic) donors are available as a separate product. These cells have demonstrated very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells. Diabetic Human Adipose Derived Stem Cells (ADSC) have been reported to differentiate down many different lineages including chondrogenic, osteogenic, adipogenic and neural. Diabetic Human Adipose Derived Stem Cells (ADSC) have been cryopreserved at primary passage.
Description: Human subcutaneous adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human subcutaneous adipose tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the subcutaneous adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The subcutaneous adipose tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
cDNA - Human Diabetic Diseased Tissue: Visceral Adipose
Description: Diseased endothelial cells are isolated from human donors that have been diagnosed with diabetes type I or type II disease. Diseased cells are grown in the same optimized media system as normal human dermal microvascular endothelial cells.
Human Dermal Microvascular EndoCells Diabetic Type II
Description: Diseased endothelial cells are isolated from human donors that have been diagnosed with diabetes type I or type II disease. Diseased cells are grown in the same optimized media system as normal human dermal microvascular endothelial cells.
Description: Diabetic Mouse Cardiac Microvascular Endothelial Cells are isolated from the heart of Diabetic (db/db) mice. Diabetic Mouse Cardiac Microvascular Endothelial Cells are cryopreserved at passage 3 and delivered frozen.
cDNA - Human Diabetic Diseased Tissue: Small Intestine: Duodenum
Bij bivariate evaluatie werd gebruikgemaakt van een chi-kwadraattest met een significantiestadium van p <0,05 en een betrouwbaarheidsinterval (BI) van 95%, en een multivariate evaluatie met behulp van een aantal logistische regressiecontroles.
Factoren die diabetes mellitus beïnvloeden, waren leeftijd> 55 jaar (OR = 5,10; 95% BI 4,42 tot 5,89; p <0,001), vrouwelijk (OR = 1,37; 95% BI 1,26 tot 1,49; p <0,001), landelijk (OR = 1,16; 95% BI 1,08 tot 1,26; p <0,001), getrouwd (OR = 1,31; 95% BI 1,07 tot 1,58; p <0,05), werkloos (OR = 1,14; 96% BI 1,05 tot 1,23; p <0,05) gewichtsproblemen (OR = 1,46; 95% BI 1,35 tot 1,58; p <0,001), hypertensie (OR = 1,68; 95% BI 1,55 tot 1,81; p <0,001) en dyslipidemie (OR = 1,53; 95% BI 1,39-1,68 ; P <0,001). CONCLUSIES Maar liefst 13% van de mensen heeft diabetes mellitus in 2013. Leeftijd, geslacht, woonruimte, werkgelegenheidsstatus, gewichtsproblemen, hypertensie en dyslipidemie zijn de bijdragende componenten aan diabetes mellitus.
